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Figure 1 | BMC Microbiology

Figure 1

From: The promoter and transcribed regions of the Leishmania tarentolae spliced leader RNA gene array are devoid of nucleosomes

Figure 1

DNA FISH localization of the SL RNA gene array and pX-tSL episome in the nucleoplasm. The genomic SL RNA gene array does not co-localize with the pX-tSL episome. A) The genomic SL RNA gene array was visualized using a green Alexafluor 488-conjugated PCR fragment that corresponded to the entire MINA SL RNA gene repeat. The genomic arrays reside in a single discrete location within the nucleus. B) Specificity control for the exon-tagged tSL RNA gene. The episome pX-tSL was visualized with an Alexafluor 488-labeled oligonucleotide, NSTAG, in transfected cells. No hybridization was observed to WT cells. C) Double staining for the SL RNA gene array and the episomal tSL RNA genes. The endogenous genes were visualized with an Alexafluor 488-conjugated PCR fragment, and the episome pX-tSL was visualized with a Tamara-labeled NSTAG oligonucleotide probe. There are examples of 1, 2, and three pX-tSL domains within the parasite nucleus.

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