Detection of NheB in culture supernatants. Western immunoblot of culture supernatants from B. cereus strains INRA AF2, NVH 883/00, NVH 391/98, NVH 0075/95 and ATCC 14579 detected using the 1C2 antibody, reactive against both NheB and Hbl component L1. Samples were collected from late log phase cultures grown at 32°C. NVH 0075/95 and ATCC 14579 were used as controls; NVH 0075/95 does not contain hbl, while ATCC 14579 produces both Nhe and Hbl. The results show that strains NVH 391/98 and INRA AF2 produce NheB.