Figure 2
From: Toxin production in a rare and genetically remote cluster of strains of the Bacillus cereus group
Detection of NheB in culture supernatants. Western immunoblot of culture supernatants from B. cereus strains INRA AF2, NVH 883/00, NVH 391/98, NVH 0075/95 and ATCC 14579 detected using the 1C2 antibody, reactive against both NheB and Hbl component L1. Samples were collected from late log phase cultures grown at 32°C. NVH 0075/95 and ATCC 14579 were used as controls; NVH 0075/95 does not contain hbl, while ATCC 14579 produces both Nhe and Hbl. The results show that strains NVH 391/98 and INRA AF2 produce NheB.