Table 3 Strains and plasmids used. Amp^R, Erm^R, Tet^R, and Km^R are ampicillin, erythromycin, tetracycline and kanamycin resistance, respectively.

Strains

E. coli K-12 BW25113

lacI^q rrnB _T14 ΔlacZ_WJ16 hsdR514 ΔaraBA-D_AH33 ΔrhaBAD_LD78

E. coli K-12 BW25113 ΔtrpE

K-12 ΔtrpE:: Km^R, defective in anthranilate synthase component I

E. coli K-12 BW25113 ΔtnaC

K-12 ΔtnaC:: Km^R, defective in the tryptophanase leader peptide

E. coli K-12 BW25113 ΔtrpL

K-12 ΔtrpL:: Km^R, defective in the trp operon leader peptide

E. coli K-12 BW25113 ΔtnaA

K-12 ΔtnaA:: Km^R, defective in tryptophan deaminase

E. coli K-12 BW25113 ΔgadA

K-12 ΔgadA:: Km^R, defective in the acid resistance gene glutamate decarboxylase A

E. coli K-12 BW25113 ΔhdeA

K-12 ΔhdeA:: Km^R, defective in acid resistance

E. coli K-12 BW25113 ΔsdiA

K-12 ΔsdiA:: Km^R, defective in sensing autoinducer-1

E. coli K-12 ATCC 25404

wild-type

E. coli K-12 ATCC25404/pGEM-T RFP

Amp^R, RFP⁺

E. coli K-12 XL1-Blue/pCM18

recA1, lac, endA1, gyrA96, thi, hsdR17, supE44, relA1/ F' proAB⁺, lacI^q, lacZ ΔM13, Tn10 Tet^R, Erm^R, GFP⁺

E. coli UT481/pCX39

Δlac-pro met pro zzz:: Tn10 thy supD r_K^- m_K^-/ftsQ2p::lacZ, Amp^R

E. coli WX2/pCX39

Δlac-pro met pro zzz:: Tn10 thy supD r_K^- m_K^- m_K^- m_K^-/ftsQ2p::lacZ, ΔsdiA:: Km^R, Amp^R

E. coli JM109

recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) F' [traD36 proAB⁺lacI^qlacZ ΔM15]

E. coli TG1

supE hsd Δ5 thi Δ(lac-proAB) F' [traD 36 proAB⁺lacI^qlacZ ΔM15]

P. aeruginosa PAO1

wild-type

P. fluorescens 2–79

wild-type (NRRL B-15132) TOM^-

P. fluorescens 2-79TOM

P. fluorescens 2–79 TOM⁺, Km^R

P. fluorescens 2-79TOM/pHKT3

TOM⁺, Km^R, Tet^R RFP⁺

P. fluorescens 2–79/pHKT3

TOM^-, Tet^R RFP⁺