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Table 3 Strains and plasmids used. AmpR, ErmR, TetR, and KmR are ampicillin, erythromycin, tetracycline and kanamycin resistance, respectively.

From: Indole is an inter-species biofilm signal mediated by SdiA

Strains and Plasmids Genotype Source
E. coli K-12 BW25113 lacIq rrnB T14 ΔlacZWJ16 hsdR514 ΔaraBA-DAH33 ΔrhaBADLD78 [67]
E. coli K-12 BW25113 ΔtrpE K-12 ΔtrpE:: KmR, defective in anthranilate synthase component I [67]
E. coli K-12 BW25113 ΔtnaC K-12 ΔtnaC:: KmR, defective in the tryptophanase leader peptide [67]
E. coli K-12 BW25113 ΔtrpL K-12 ΔtrpL:: KmR, defective in the trp operon leader peptide [67]
E. coli K-12 BW25113 ΔtnaA K-12 ΔtnaA:: KmR, defective in tryptophan deaminase [67]
E. coli K-12 BW25113 ΔgadA K-12 ΔgadA:: KmR, defective in the acid resistance gene glutamate decarboxylase A [67]
E. coli K-12 BW25113 ΔhdeA K-12 ΔhdeA:: KmR, defective in acid resistance [67]
E. coli K-12 BW25113 ΔsdiA K-12 ΔsdiA:: KmR, defective in sensing autoinducer-1 [67]
E. coli K-12 ATCC 25404 wild-type ATCC
E. coli K-12 ATCC25404/pGEM-T RFP AmpR, RFP+ this work
E. coli K-12 XL1-Blue/pCM18 recA1, lac, endA1, gyrA96, thi, hsdR17, supE44, relA1/ F' proAB+, lacIq, lacZ ΔM13, Tn10 TetR, ErmR, GFP+ this work
E. coli UT481/pCX39 Δlac-pro met pro zzz:: Tn10 thy supD rK- mK-/ftsQ2p::lacZ, AmpR [31]
E. coli WX2/pCX39 Δlac-pro met pro zzz:: Tn10 thy supD rK- mK- mK- mK-/ftsQ2p::lacZ, ΔsdiA:: KmR, AmpR [31]
E. coli JM109 recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) F' [traD36 proAB+lacIqlacZ ΔM15] [68]
E. coli TG1 supE hsd Δ5 thi Δ(lac-proAB) F' [traD 36 proAB+lacIqlacZ ΔM15] [55]
P. aeruginosa PAO1 wild-type T. McDermott
P. fluorescens 2–79 wild-type (NRRL B-15132) TOM- [28]
P. fluorescens 2-79TOM P. fluorescens 2–79 TOM+, KmR [28]
P. fluorescens 2-79TOM/pHKT3 TOM+, KmR, TetR RFP+ this work
P. fluorescens 2–79/pHKT3 TOM-, TetR RFP+ this work