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Table 1 Purification of alanine racemase from Streptococcus pneumoniae

From: Purification and preliminary crystallization of alanine racemase from Streptococcus pneumoniae

 

Volume [ml]

Protein Conc [mg/ml]

Total Protein [mg]

Specific Activity [u/mg]

Total Activity [u]

Crude Extract

90.00

12.50

1125.00

5.10

5736.54

Supernatant after 20 % AmSO4 precipitation

95.00

8.90

845.50

6.33

5358.91

Resuspended pellet after 60 % AmSO4 precipitation

35.00

22.50

787.50

6.74

5311.40

Before Q-Sepharose

45.00

13.00

585.00

8.16

5143.09

After Q-Sepharose

24.00

3.80

91.20

45.87

4174.49

After HIC

10.00

7.00

70.00

49.91

3556.27

Before Superdex

8.00

3.30

26.40

64.09

1692.09

After Superdex

0.50

50.00

25.00

65.81

1645.23