Similarity of MelF to the bioluminescence protein luciferase. Alignment of MelF with LuxA and LuxB proteins from bioluminescent bacteria and the concensus (pfam00296) (A) and a dendogram (B) showing the relationship between them. Numbers to the left of the alignment indicate the position within each protein and above indicate the position within pfam00296. Residues that match the concensus are shown outlined by boxes. Asterixes above alignment indicate conserved regions thought to be involved in enzyme activity. Abbreviations for aligned proteins and their accession numbers are as follows: melF, M. marinum MelF (AAV32084); pfam00296, luciferase-like monooxygenases conserved domain; luxAPle, Photobacterium leiognathi LuxA (P29238); luxAPlu, Photorhabdus luminescens LuxA (AAK98554); luxASha, Shewanella hanedai LuxA (BAB40796); luxAVfi, Vibrio fishcheri LuxA (AAD48477); luxAVha, Vibrio harveyi LuxA (CAA41597); luxBPle, Photobacterium leiognathi LuxB (P09141); luxBPlu, Photorhabdus luminescens LuxB (AAK98555); luxBPph, Photobacterium phosphoreium LuxB (P12744); luxBSha, Shewanella hanedai LuxB (BAB40797); luxBVfi, Vibrio fishcheri LuxB (AAD48478); luxBVha, Vibrio harveyi LuxB (AAA88686). The dendogram was rendered from the alignment using MegAlign (DNASTAR). Tree length is shown to the left of the log scale ruler beneath the dendogram.