Figure 4
Monitoring the expression of Cpn0146, 0147, 0284 and 0285 proteins during chlamydial infections. HeLa cells infected with C. pneumoniae AR39 organisms for various periods of time as indicated on top of the figures were subjected to immunostaining with the anti-Cpn0146 (clone 3G2; panels a-f), anti-Cpn0147 (clone 2G4; panels g-n), anti-Cpn0186 (IncA; clone 2B12.1; o-v), anti-Cpn0284 (pAb; w-dd) and anti-Cpn0285 (pAb; ee-ll) antibodies as listed along the left side of the figure (red). The rabbit antibody R12AR39 was used to visualize the inclusions (green) and Hoechst dye for DNA (blue). The images were acquired using the conventional fluorescence microscope. Note that Cpn0147 protein was first detected 6 hours (panel h, white arrow), IncA 12 hours (q, white arrow), Cpn0146, 0284 & 0285 24 hours (panels z & hh respectively, white arrows) after infection with C. pneumoniae.