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Table 3 Viability of M. mycoides subsp. mycoides SC cell suspensions upon starvation at 37°C in medium-free buffers supplemented with 5 μM sucrose or 5 mM glucose

From: β-D-Glucoside utilization by Mycoplasma mycoides subsp. mycoides SC: possible involvement in the control of cytotoxicity towards bovine lung cells

incubation time (h)

CFUa

 

HEPES

glucose

sucrose

strain 8740

0

≈ 107

≈ 107

≈ 107

4

61 × 105 (61%)

82 × 105 (82%)

93 × 105 (93%)

18

165 × 102 (0.16%)

21 × 103 (0.21%)

123 × 104 (12.3%)

strain T1/44

0

≈ 107

≈ 107

≈ 107

4

77 × 105 (77%)

87 × 105 (87%)

83 × 105 (83%)

18

131 × 102 (0.13%)

35 × 103 (0.35%)

104 × 102 (0.10%)

  1. a For all assays, the original CFU (at time zero) was considered as being ≈ 107 (10 μl of a suspension containing ≈ 109 CFU/ml). CFUs are reported as number of colonies formed in a specific sector of the plate X dilution factor. Numbers in parentheses represent the percentages in relation to the initial 107 CFU.