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Table 3 Regulation of sifA by ssrAB.

From: Systematic analysis of the regulation of type three secreted effectors in Salmonella enterica serovar Typhimurium

  LB MgM
WT sifA-lac 5.5 ± 1.6 26.3 ± 4.8
MM sifA-lac 3.6 ± 0.5 16.8 ± 2.9
MM sifA-lac + p-ssrB 4.8 ± 0.8 29.6 ± 6.9
MM sifA-lac + pWSK29 3.8 ± 0.5 15 ± 5.6
Δ ssrA sifA-lac 3.8 ± 1.1 14.4 ± 2.7
Δ ssrB sifA-lac 3.9 ± 1.4 14.2 ± 2.5
Δ ssrAB sifA-lac 2.7 ± 2.4 15.6 ± 2.6
  1. The β-galactosidase activities of strains harbouring a sifA-lac fusion were measured using ONPG. Each value represents the mean Miller units of at least three experiments ± standard deviation. WT, wild type 14028; MM, multi-mutant [Δ(avrA-invH)1 ssrB::cat sirA4::hyg].