Anti-RocF Western on minimally-passaged wild type H. pylori. Proteins were separated by SDS-PAGE using a 12% resolving gel and 10 μg of protein loaded per lane and analyzed by anti-RocF antibodies. Only a subset of strains is shown. J104-L, low arginase activity strain minimally passaged; J104-H, high arginase activity strain passaged for seven to nine days; SS1-L, low arginase activity strain passaged for seven to nine days; SS1-H, high arginase activity strain minimally-passaged. Numbers below the top two blots represent the arginase specific activity (rounded to nearest 100; pmol L-ornithine/min/mg protein ± standard deviation) of the extract used for the blot.