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Table 4 Complete assignment of the NMR spectra of the mono-O-acetylated polysaccharide

From: The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region

NRT36 Mono-O-Acetyl ~50°C

H-1

H-2

H-3

H-4

H-5

H-6, H-6'

 

C-1

C-2

C-3

C-4

C-5

C-6

A: α-D-GlcA

5.15

3.63

3.67

3.51

3.95

-

 

101.9

72.7

73.8

72.7

74.1

176.7

B: α-D-GlcNAc

4.99 (5.01)

3.91 (4.05)

3.99 (5.27)

3.63 (3.87)

4.14

4.09, 3.71

 

94.9 (94.1)

53.9 (52.6)

71.6 (74.3)

80.3

68.6

68.4

C: β-L-Rha

5.04

5.53

3.80

3.48

3.49

1.36

 

99.9

69.9

76.3

71.6

73.2

17.6

D: β-D-GlcNAc

4.39

3.77

3.66

3.69

3.43

3.90, 3.88

 

102.1

56.3

74.7

77.8

75.1

61.7

a.) Shifts enclosed in parentheses are those of the 3-O-acetylated B residue in the native polysaccharide.

assignment of NAc's, OAc's

NH

C = O

CH 3

   

B: α-D-GlcNAc

7.79

174.9

22.93; 2.03

   

D: β-D-GlcNAc

8.09

175.2

23.19; 2.08

   

C: β-L-Rha OAc

 

173.9

21.26; 2.21

  Â