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Figure 8 | BMC Microbiology

Figure 8

From: Integration of regulatory signals through involvement of multiple global regulators: control of the Escherichia coli gltBDF operon by Lrp, IHF, Crp, and ArgR

Figure 8

Interactions between ArgR and the gltBDF promoter region. A. The ArgR-binding sequences upstream of gltBDF (top two lines) compared to the symmetrical consensus ArgR-binding site [64]. Matches to the consensus are underlined. W = A or T, and N = A, C, G or T. B. Gel mobility shift analysis of ArgR binding to the region -406 to +246 of glt DNA. L-arginine was present in the reactions, the gel and running buffer (see Methods). C. DNase I protection assay of the region -462 to +161 bp upstream of gltBDF, labelled at the -462 end of the partner strand. The first 4 lanes represent a sequencing ladder of the partner strand in this region. ArgR concentrations of 0, 3.13, 6.25, 12.5, and 25 nM were used, and 5 mM L-arginine was present.

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BMC Microbiology

ISSN: 1471-2180

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