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Figure 4 | BMC Microbiology

Figure 4

From: Integration of regulatory signals through involvement of multiple global regulators: control of the Escherichia coli gltBDF operon by Lrp, IHF, Crp, and ArgR

Figure 4

Masses of polypeptides associated with gltB DNA in vitro. Surface-enhanced laser desorption and ionization (SELDI; Ciphergen Biosystems) was used to identify proteins from whole-cell E. coli extracts binding to biotin-labeled gltB DNA (from -406 to +246). The DNA was attached to streptavidin-coated chips, incubated with the extracts, and washed prior to mass analysis. Parts A and B show different mass ranges. The vertical arrows indicate the theoretical positions of the indicated polypeptides; in each case the mass shown assumes removal of the N-terminal methionine.

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