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Table 1 Strains used in the study.

From: Importance of c-Type cytochromes for U(VI) reduction by Geobacter sulfurreducens

Strain name

Genotype

Genbank accession # of deleted gene(s)

Predictedb or confirmedc Transcription unit(s) (5' to 3')

Source or reference

DL1 (ATCC 51573)

Wild type

-

-

[53]

DL5

omcC::kan

GI-39997825

1) orf1orf2omcCc

2) omcCc

[25] [54]

DL6a

omcB::cam

GI-39997831

1) orf1orf2omcBc

2) omcBc

[25] [54]

DLMC8a

omcE::kan

GI-39995725

1) omcEb

[35]

DLBK01a

omcF::kan

GI-39997527

1) gsu2433, omcF, gsu2431, gsu2430c

[24]

DLMC6

gsu3332::kan

GI-39998421

1) gsu3332, gsu3331, gsu3330b

This work

DLMC5

gsu1334::kan

GI-39996436

1) gsu1336, gsu1334b

This work

DLLD2a

ppcE::kan

GI-39996859

1) gsu1758, gsu1759, ppcEb

[28]

DLLD4a

ppcBppcC::kan

GI-39995472

GI-39995473

1) ppcB, gsu0363b

2) ppcCb

This work (L. Didonato, personal communication)

DL3a

ppcA::kan

GI-39995719

1) ppcAb

[27]

DLLD3a

ppcD::kan

GI-39996127

1) ppcDb

[28] (C. Leang, personal communication)

DLMC7

gsu0616::kan

GI-39995723

1)gsu0615, gsu0616, gsu0617b

This work

DL1-MacA

macA::kan

GI-39995574

1) macAc

[26]

  1. aStrains for which impairment in growth via Fe(III) reduction was complementation complemented by expression of the relevant cytochrome gene in trans. In the case of strain DLMC8, impairment in growth on Fe(III) oxide was complemented by expression of OmcE in trans. In all other cases Fe(III) citrate phenotypes were complemented. The Fe(III) citrate reduction phenotype of the double mutant, DLLD4 was complemented by expressing ppcB in trans.
  2. bTranscription units were predicted with the FGENESB software package (Softberry Inc., Mt. Kisco, NY) as previously described [55]. The numbers 1) and 2) indicate distinct, predicted transcripts.
  3. cTranscription units have been experimentally confirmed by Northern blot. The numbers 1) and 2) refer to distinct, experimentally confirmed transcripts.