Gene | Primer | Oligonucleotide sequence (5'-3') | Fragment size (bp) | Annealing temperature | Primer Coordinatesa | Accession number |
---|---|---|---|---|---|---|
stx1 | VT1-A | CGCTGAATGTCATTCGCTCTGC | 302 | 55°C | 113–134 | M17358 |
VT1-B | CGTGGTATAGCTACTGTCACC | 394–414 | ||||
stx 2 | VT2-A | CTTCGGTATCCTATTCCCGG | 516 | 55°C | 50–69 | M59432 |
VT2-B | CTGCTGTGACAGTGACAAAACGC | 543–565 | ||||
ehxA | HlyA1 | GGTGCAGCAGAAAAAGTTGTAG | 1.551 | 60°C | 238–259 | X79839 |
HlyA4 | TCTCGCCTGATAGTGTTTGGTA | 1767–1788 | ||||
eae b | EAE-1 | GGAACGGCAGAGGTTAATCTGCAG | 346 | 55°C | 631–654 | AF022236 |
EAE-2 | GGCGCTCATCATAGTCTTTC | 957–976 | ||||
eae-α1 | EAE-FB | AAAACCGCGGAGATGACTTC | 820 | 60°C | 1909–1928 | AF022236 |
EAE-A | CACTCTTCGCATCTTGAGCT | 2709–2728 | ||||
eae-α2 | IH2498aF | AGACCTTAGGTACATTAAGTAAGC | 517 | 60°C | 2099–2122 | AF530555 |
IH2498aR | TCCTGAGAAGAGGGTAATC | 2597–2615 | ||||
eae-β1 | B1F | CACAATTAATGCACCGGGT | 241 | 55°C | 2499–2517 | AF453441 |
B1R | GCTTGATACACCTGATGACT | 2720–2739 | ||||
eae-ξR/β2B | B2F | TGAAGGGGGGAACCCCTGTG | 564 | 62°C | 2054–2073 | AF530556 |
B2R | TTTCTTTTGACTGTGCTAAAGC | 2596–2617 | AJ715407 | |||
eae-δ/κ/β2O | EAE-FB | AAAACCGCGGAGATGACTTC | 830 | 60°C | 1909–1928 | U66102 |
EAE-D | CTTGATACACCCGATGGTAAC | 2718–2738 | ||||
eae-γ1 | EAE-FB | AAAACCGCGGAGATGACTTC | 804 | 60°C | 1909–1928 | AF071034 |
EAE-C1 | AGAACGCTGCTCACTAGATGTC | 2691–2712 | ||||
eae-θ/γ2 | EAE-C2F | AGAACGTTACTGGTGACTTA | 414 | 58°C | 2303–2322 | AF025311 |
EAE-C2R | CTGATATTTTATCAGCTTCA | 2697–2716 | ||||
eae-ε1 | EAE-FB | AAAACCGCGGAGATGACTTC | 722 | 66°C | 1909–1928 | AF116899 |
LP5 | AGCTCACTCGTAGATGACGGCAAGCG | 2605–2630 | ||||
eae-νR/ε2 | EAE-E2F | AATACAGAAGTTAAGGCAT | 378 | 58°C | 2230–2248 | AF530554 |
EAE-E2R | ACGACCACTATTCATTTC | 2590–2607 | ||||
eae-ζ | Z1 | GGTAAGCCGTTATCTGCC | 206 | 62°C | 2062–2079 | AF449417 |
Z2 | ATAGCAAGTGGGGTGAAG | 2250–2267 | ||||
eae-η1/η2 | EAE-FB | AAAACCGCGGAGATGACTTC | 702 | 60°C | 1899–1918 | AJ308550 |
ETA-B | TAAGCGACCACTATTCGTG | 2582–2600 | ||||
eae-η1 | ETA-FN | CGCTTTGTTTAATGCCGATAAA | 410 | 62°C | 1074–1095 | AJ308550 |
ETA-RN | GACTGCGTAATGCACTG | 1467–1483 | ||||
eae-ι1 | EAE-FB | AAAACCGCGGAGATGACTTC | 651 | 55°C | 1909–1928 | AJ308551 |
IOTA-B | GTCATATTTAACTTTTACACTA | 2538–2559 | ||||
eae-μR/ι2, | Iota2-F | CTGGTAAAGCGATAGTCAAAC | 936 | 58°C | 1850–1870 | AF530553 |
Iota2-R | GCGTTTTTGAAGAAACATTTTGC | 2763–2785 | ||||
eae-λ | 68.4F | CGGTCAGCCTGTGAAGGGC | 370 | 64°C | 2061–2079 | AJ715409 |
68.4R | AATACCGGAAGAGGCATCTAT | 2410–2430 | ||||
eae-μB | EAE-FB | AAAACCGCGGAGATGACTTC | 655 | 60°C | 1909–1928 | AJ705049 |
FV373R | ACTCATCATAATAAGCTTTTTGG | 2541–2563 | ||||
eae-νB | IH1229aF | CACAGCTTACAATTGATAACA | 311 | 60°C | 2255–2275 | AJ705050 |
IH1229aR | CTCACTATAAGTCATACGACT | 2545–2565 | ||||
eae-ξB | EAE-FB | AAAACCGCGGAGATGACTTC | 468 | 66°C | 1909–1928 | AJ705051 |
B49R | ACCACCTTTAGCAGTCAATTTG | 2355–2376 | ||||
eae-ο | H2997fF | AGCGTTAGCAATGCCGCAGTTGAT | 271 | 60°C | 2203–2226 | AJ876647 |
IH2997fR | CAACGGTAATTGTTGTTTCC | 2454–2473 | AJ876648 |