Characterization of the Trichomonas vaginalis surface-associated AP65 and binding domain interacting with trichomonads and host cells

Table 1 Primers¹ used for cloning of ap65-3 gene

Clone	Primer sequence for AP65-3	Plasmid
5' c1 Bgl II	GGGGAGATCTGATGCTCGCATCTTCAGTCG	pET-26b(+)
3' c1 Bgl II	GGGGAGATCTGGCCAGCCGTGGTAGAGTG	pET-26b(+)
5' c2 Bgl II	GGGGAGATCTGATGACCCTCCTTCAGG	pET-26b(+)
3' c2 Bgl II	GGGGAGATCTGGCCAGCCGTGGTAGAGTG	pET-26b(+)
5' c3 Bgl II	GGGGAGATCTGGGGAGATTCCTCTTCACACA	pET-26b(+)
3' c3 Bgl II	GGGGAGATCTGGGAAGCAGTTGCAGCGCCAG	pET-26b(+)
5' c4 Bgl II	GGGGAGATCTAATCCTCGCCGACCCA	pET-26b(+)
3' c4 Bgl II	GGGGAGATCTACACCCTCAAGGACGGAG	pET-26b(+)
5' c5 Bgl II	GGGGAGATCTGATGGTCCATGCTGACCGTA	pET-26b(+)
3' c5 Bgl II	GGGGAGATCTAGGTCCTTGAGCTCTGTG	pET-26b(+)
5' FL NdeI	GTCCAGCATATGATGCTCGCATCTTCAGTCG	pBS-ap65-HA
3' FL Asp718	GTCCACGGTACCATAGTAGAGTTGCTCGTATTC	pBS-ap65-HA

¹ These primers were used for the cloning of overlapping fragments and full-length of the ap65-3 gene in the pET-26b(+) vector (Novagen EMD Chemicals Inc) in E. coli and pBS-HA vector in T. vaginalis [16, 23].