Recombinant subclone c1 binds to VECS and T. vaginalis. Part A shows the immunoblot of chemically-stabilized MS-74 VECs (A1) and T. vaginalis (A2) that were incubated with 10 μg of each purified recombinant clone for 1 h at RT. Cells were processed for the ligand assay (Materials and Method). After SDS-PAGE using 10% acrylamide gels, proteins were transferred to PVDF membranes and probed with anti-Penta-His IgG mAb. Only recombinant clone c1 bound to fixed cells in the ligand assay for both cell types. Part B shows a Coomassie-blue stained gel containing 1 μg of each recombinant clones to illustrate that equal amounts of recombinant clones were added to cells in the ligand assays.