Representative experiment showing pretreatment of parasites with cycloheximide and/or trypsin abolishes adherence to VECs. T. vaginalis were incubated in the presence or absence of cycloheximide (C), trypsin (T) or the combination of both (C+T) for 30 and 15 min. Treatment and adherence assays were performed as described in Materials and Methods. This graph is a representative experiment of three independent experiments with eight samples per experiment. For each experiment, statistical analysis using the t-test was performed, and error bars indicate the standard deviation for each condition. An asterisk above the bars indicates the p values were all less than 0.05.