Table 4 Overview of the primer sequences and PCR conditions as used in this study
Specificity | Name | Primer sequence (5'-3') | 16S rDNA positiona (5'-3') | Cycling conditions | Reference |
|---|---|---|---|---|---|
A. vaginae | ATOVAGRT3Fw | GGTGAAGCAGTGGAAACACT | 1006–1025 | 10' 95°C, (15" 95°C, 20" 62°C, 40" 72°C) × 40 | This study |
|  | ATOVAGRT3Rev | ATTCGCTTCTGCTCGCGCA | 1282–1265 |  |  |
G. vaginalis | F-GV1 | TTACTGGTGTATCACTGTAAGG | 16S-23S spacer | 10' 95°C, (45" 94°C, 45" 55°C, 45" 72°C) × 50 | [27] |
| Â | R-GV3 | CCGTCACAGGCTGAACAGT | 16S-23S spacer | Â | Â |
L. crispatus | LcrisF | AGCGAGCGGAACTAACAGATTTAC | 65–89 | 10' 95°C, (15" 95°C, 1' 60°C) × 40 | [26] |
|  | LcrisR | AGCTGATCATGCGATCTGCTT | 205–185 |  |  |
L. gasseri | LactoF | TGGAAACAGRTGCTAATACCG | 157–177 | 10' 95°C, (15" 95°C, 1' 60°C) × 40 | [26] |
|  | LgassR | CAGTTACTACCTCTATCTTTCTTCACTAC | 470–442 |  |  |
L. iners | InersFw | GTCTGCCTTGAAGATCGG | 70–85 | 10' 95°C, (1' 95°C, 1' 55°C, 1' 65°C) × 35 | This study |
|  | InersRev | ACAGTTGATAGGCATCATC | 228–210 |  |  |
L. jensenii | LABJENTR2Fw | CCTTAAGTCTGGGATACCATT | 117–137 | 10' 95°C, (15" 95°C, 10" 54°C, 30" 72°C) × 40 | This study |
|  | LABJENRT2Rev | ACGCCGCCTTTTAAACTTCTT | 207–187 |  |  |