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Table 3 Alignment of the 16S rRNA gene primers of A. vaginae with the sequences of the described Atopobium species

From: Quantitative determination by real-time PCR of four vaginal Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. iners

Forward primer (position 1006–1025)

5'

                    

3'

A. vaginae

 

G

G

T

G

A

A

G

C

A

G

T

G

G

A

A

A

C

A

C

T

 

A. fossor

 

.

.

.

.

.

.

.

.

G

.

C

.

.

.

.

.

.

G

T

C

 

A. minutum

 

.

.

.

.

.

.

.

.

G

.

C

.

.

.

.

.

.

G

T

C

 

A. parvulum

 

.

.

.

.

.

.

.

.

G

.

C

.

.

.

.

.

.

G

T

C

 

A. rimae

 

.

.

.

.

.

.

.

.

G

.

C

.

.

.

.

.

.

G

T

C

 

Reverse primer (position 1282–1265)

5'

                   

3'

 

A. vaginae

 

A

T

T

C

G

C

T

T

C

T

G

C

T

C

G

C

G

C

A

  

A. fossor

 

.

.

.

G

.

.

.

.

A

C

T

T

.

.

.

.

A

A

G

  

A. minutum

 

.

.

.

G

.

.

.

.

A

C

T

T

.

.

.

.

A

A

G

  

A. parvulum

 

.

.

.

.

.

.

.

C

A

C

C

.

.

.

.

.

.

G

G

  

A. rimae

 

.

.

.

.

.

.

.

C

G

C

C

.

.

T

.

.

.

G

G

  
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BMC Microbiology

ISSN: 1471-2180

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