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Table 1 Alignment of the 16S rRNA gene primers of L. iners with the sequences of other important vaginal lactobacilli

From: Quantitative determination by real-time PCR of four vaginal Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. iners

Forward primer (position 70–85) 5'                    3'  
L. iners   G T C T G C C T T G A A G A T C G G   
L. crispatus   A C . . . . . C C A T . . T C T . .   
L. gasseri   A C . . . . . C A A G . .   C T . .   
L. jensenii   A C . . . . . C . T . . . T C T . .   
L. vaginalis   A C . . . . . C . . . . . C G G . .   
Reverse primer (position 228–210) 5'                     3'
L. iners   A C A G T T G A T A G G C A T C A T C  
L. crispatus   . . . C . . A G C . C T . . . . G . T  
L. gasseri   . . . C . . A G C . C T . . . . G . T  
L. jensenii   . . . C . . A G C . C T . . . . G . T  
L. vaginalis   . . . C C . A G C . C T . . . . G . T