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Table 1 Alignment of the 16S rRNA gene primers of L. iners with the sequences of other important vaginal lactobacilli

From: Quantitative determination by real-time PCR of four vaginal Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. iners

Forward primer (position 70–85)

5'

                  

3'

 

L. iners

 

G

T

C

T

G

C

C

T

T

G

A

A

G

A

T

C

G

G

  

L. crispatus

 

A

C

.

.

.

.

.

C

C

A

T

.

.

T

C

T

.

.

  

L. gasseri

 

A

C

.

.

.

.

.

C

A

A

G

.

.

 

C

T

.

.

  

L. jensenii

 

A

C

.

.

.

.

.

C

.

T

.

.

.

T

C

T

.

.

  

L. vaginalis

 

A

C

.

.

.

.

.

C

.

.

.

.

.

C

G

G

.

.

  

Reverse primer (position 228–210)

5'

                   

3'

L. iners

 

A

C

A

G

T

T

G

A

T

A

G

G

C

A

T

C

A

T

C

 

L. crispatus

 

.

.

.

C

.

.

A

G

C

.

C

T

.

.

.

.

G

.

T

 

L. gasseri

 

.

.

.

C

.

.

A

G

C

.

C

T

.

.

.

.

G

.

T

 

L. jensenii

 

.

.

.

C

.

.

A

G

C

.

C

T

.

.

.

.

G

.

T

 

L. vaginalis

 

.

.

.

C

C

.

A

G

C

.

C

T

.

.

.

.

G

.

T

 
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BMC Microbiology

ISSN: 1471-2180

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