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Table 1 Immunoreactivity of the S. pneumoniae IgA1 protease antigen fragments PaP

From: Identification of a human immunodominant B-cell epitope within the immunoglobulin A1 protease of Streptococcus pneumoniae

Serum no.

IgG Rec-ELISA (OD) PbP

 

GST-SP4

GST-epiA

GST-epiB

GST

PP1

2.47

2.62

2.13

0.26

PP2

2.19

1.63

0.45

0.10

S49

1.40

0.48

0.19

0.32

S50

1.74

1.52

0.23

0.24

S52

2.75

0.57

0.12

0.21

S53

0.42

0.23

0.28

0.08

S54

0.32

0.18

0.05

0.05

S58

1.80

0.94

0.75

0.06

S61

0.15

0.42

0.23

0.06

S62

0.44

1.47

0.29

0.13

S66

0.90

0.70

0.12

0.06

S67

0.31

0.39

0.25

0.25

S68

0.13

0.19

0.08

0.11

S69

1.22

0.24

0.12

0.11

S73

0.70

0.95

0.13

0.07

S74

0.44

0.42

0.34

0.06

S75

0.60

0.25

0.13

0.10

S76

1.64

1.72

0.43

0.08

S81

0.72

0.49

0.14

0.09

S82

0.06

0.07

0.05

0.05

S83

0.40

0.76

0.21

0.13

S84

1.01

0.73

0.16

0.07

S85

0.37

0.18

0.24

0.08

S87

0.11

0.22

0.15

0.13

S88

1.27

1.15

1.47

0.07

S89

0.46

0.61

1.34

0.15

S90

2.81

2.74

0.46

0.13

S92

0.90

0.90

0.44

0.05

S94

0.31

0.26

0.47

0.09

S96

0.52

0.54

0.60

0.06

S98

1.09

0.58

0.12

0.05

S100

2.01

0.64

0.89

0.06

S101

1.84

0.76

0.14

0.08

S102

0.56

0.22

0.10

0.04

S103

0.73

0.41

0.15

0.05

S104

0.39

0.40

0.49

0.03

S106

0.85

0.81

0.23

0.21

S107

0.67

0.30

0.27

0.05

S108

1.16

0.30

0.16

0.05

S109

0.55

0.17

0.11

0.05

S110

0.64

0.55

0.30

0.05

S111

0.75

0.46

1.31

0.05

S112

0.13

0.11

0.17

0.04

S113

1.52

0.53

0.34

0.07

S114

0.19

0.20

0.12

0.05

S115

1.66

1.76

1.90

0.04

S116

1.15

0.37

0.61

0.07

S117

2.07

1.90

2.20

0.18

  1. Pa PSerum samples from patients with pneumococcal pneumonia (PP1 and PP2) and from healthy adults (S49 to S117) were analyzed by IgG Rec-ELISA with individual recombinant proteins.
  2. Pb PThe cutoff values were determined for each serum as two times the OD readings obtained with the GST. Boldface type, OD values greater than the cutoff values.
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BMC Microbiology

ISSN: 1471-2180

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