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Table 2 Sequences of primers used for the V-BiA-RE nanoplex PCR.

From: A nanoplex PCR assay for the rapid detection of vancomycin and bifunctional aminoglycoside resistance genes in Enterococcus species

Gene Primer Name 5'--------------------------------3' GenBank Accession Number Product Size
16S rRNA 16SrRNA-F AGG GGA TAA CAC TTG GAA ACA AB015233 1178 bp
  16SrRNA-R TTC GCG ACT CGT TG TAC TTC   
ddl E. faecalis Elis-F2 GGC CCT CTT TTA TCT GAA CGA U00457 734 bp
  Elis-R3 GCG ACT TAA GCC ACT TCC AT   
ddl E. faecium Ecium-F CGC AGA GCA TGA AGT GTC CA AF550665 557 bp
  Ecium-R2 CTT CTC GGT TTT CTG CTT TTG TA   
Van A vanA-F TTG GGG GTT GCT CAG AGG AG X56895 931 bp
  vanA-R CTT CGT TCA GTA CAA TGC GG   
Van B vanB-F AAT GCG GGG AGG ATG GTG CG AF550667 446 bp
  vanB-R GAT GCG GAA GAT ACC GTG GC   
Van C vanC-F2 GCA GGT TCT GCC TTA TGT ATG AA AF162694, AY033764, L29638 339 bp
  vanC-R ATG AAA TGG CGT CAC AAG CA   
Van D vanD-F CGT ATG TGG GAT GCG ATA TTC AA AF130997 230 bp
  vanD-R2 CTT CGA TTG CTG CCT GCA GTT   
aac A- aph D acph-F1L GAT TTG CCA GAA CAT GAA TTA CAC GA AY602207 156 bp
  acph-R1L CAT AAC CAC TAC CGA TTA TTT CAA T   
Internal Control ( ctx A) IC-F AAC TCA GAC GGG ATT TGT TAG GC AF510996 300 bp
  IC-R TCT CTG TAG CCC CTA TTA CGA TGT