Table 2 Sequences of primers used for the V-BiA-RE nanoplex PCR.
Gene | Primer Name | 5'--------------------------------3' | GenBank Accession Number | Product Size |
|---|---|---|---|---|
16S rRNA | 16SrRNA-F | AGG GGA TAA CAC TTG GAA ACA | AB015233 | 1178 bp |
16SrRNA-R | TTC GCG ACT CGT TG TAC TTC | |||
ddl E. faecalis | Elis-F2 | GGC CCT CTT TTA TCT GAA CGA | U00457 | 734 bp |
Elis-R3 | GCG ACT TAA GCC ACT TCC AT | |||
ddl E. faecium | Ecium-F | CGC AGA GCA TGA AGT GTC CA | AF550665 | 557 bp |
Ecium-R2 | CTT CTC GGT TTT CTG CTT TTG TA | |||
Van A | vanA-F | TTG GGG GTT GCT CAG AGG AG | X56895 | 931 bp |
vanA-R | CTT CGT TCA GTA CAA TGC GG | |||
Van B | vanB-F | AAT GCG GGG AGG ATG GTG CG | AF550667 | 446 bp |
vanB-R | GAT GCG GAA GAT ACC GTG GC | |||
Van C | vanC-F2 | GCA GGT TCT GCC TTA TGT ATG AA | AF162694, AY033764, L29638 | 339 bp |
vanC-R | ATG AAA TGG CGT CAC AAG CA | |||
Van D | vanD-F | CGT ATG TGG GAT GCG ATA TTC AA | AF130997 | 230 bp |
vanD-R2 | CTT CGA TTG CTG CCT GCA GTT | |||
aac A- aph D | acph-F1L | GAT TTG CCA GAA CAT GAA TTA CAC GA | AY602207 | 156 bp |
acph-R1L | CAT AAC CAC TAC CGA TTA TTT CAA T | |||
Internal Control ( ctx A) | IC-F | AAC TCA GAC GGG ATT TGT TAG GC | AF510996 | 300 bp |
IC-R | TCT CTG TAG CCC CTA TTA CGA TGT |