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Table 1 Bacterial species and strains used in this study and results of V-BiA-RE nanoplex PCR.

From: A nanoplex PCR assay for the rapid detection of vancomycin and bifunctional aminoglycoside resistance genes in Enterococcus species

No. Reference strains 16S rRNAa ddl-E. faecalis ddl-E. faecium van A van B van C vanD aac A-aph Db Internal control
1. E. faecium LMG 16004c + - + + - - - - +
2. E. durans LMG 16172c + - - + - - - - +
3. E. faecium LMG 16192c + - + + - - - - +
4. E. faecalis LMG 8222c + + - - - - - - +
5. E. faecalis LMG 17122c + + - - - - - - +
6. E. faecalis LMG 16216c + + - - + - - + +
7. E. faecium LMG 16200c + - + - - - - - +
8. E. raffinosus LMG 12172c + - - - - - - - +
9. E. mundti LMG 12308c + - - - - - - - +
10. E. hirae LMG 6399c + - - - - - - - +
11. E. avium, LMG 10744c + - - - - - - - +
12. E. faecium BM 4339 d + - + - - - + + +
13. E. casseliflavus e + - - - - + - - +
14. S. aureus f - - - - - - - - +
15. Streptococcus spp. Group Af - - - - - - - - +
16. Streptococcus spp. Group Bf - - - - - - - - +
17. Streptococcus spp. Group Gf - - - - - - - - +
18. Streptococcus spp. Group Ff - - - - - - - - +
19. Bacillus spp.f - - - - - - - - +
20. Listeria spp.f - - - - - - - - +
21. Corynebacterium spp.f - - - - - - - - +
22. Gardnerella spp.f - - - - - - - - +
23. Lactobacillus spp.f - - - - - - - - +
24. E. coli (EHEC)f - - - - + - - - +
25. E. coli (EPEC)f - - - - - - - - +
26. E. coli (ETEC)f - - - - + - - - +
27. V. cholerae (O1 classical)f - - - - - - - - +
28. V. cholerae O139 f - - - - - - - - +
29. V. mimicus f - - - - - - - - +
30. V. cincinnatiensis f - - - - - - - - +
31. V. furnissii f - - - - - - - - +
32. V. parahaemolyticus f - - - - - - - - +
33. S. typhi f - - - - - - - - +
34. S. sonnei f - - - - - - - - +
35. S. enterica f - - - - - - - - +
36. S. dysenteriae f - - - - - - - - +
37. S. boydii f - - - - - - - - +
38. C. freundii f - - - - - - - - +
39. Y. enterocolitica f - - - - - - - - +
40. P. mirabilis f - - - - - - - - +
41. P. aeruginosa f - - - - - - - - +
42. K. pneumoniae f - - - - - - - - +
43. P. Shigelloides f - - - - - - - - +
  1. aEnterococcus genus
  2. b Bifunctional aminoglycoside resistant genotype
  3. c Reference strains from Belgian Co-ordinated Collections of Micro-organisms (BCCM), Ghent, Belgium
  4. d Kindly provided by Professor. Patrice Courvalin and Dr. Bruno Perichon, Institut Pasteur, Paris, France
  5. e Obtained from Institute for Medical Research, Malaysia
  6. f Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia. '+' is positive; '-' is negative by V-BiA-RE PCR