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Table 1 Bacterial species and strains used in this study and results of V-BiA-RE nanoplex PCR.

From: A nanoplex PCR assay for the rapid detection of vancomycin and bifunctional aminoglycoside resistance genes in Enterococcus species

No.

Reference strains

16S rRNAa

ddl-E. faecalis

ddl-E. faecium

van A

van B

van C

vanD

aac A-aph Db

Internal control

1.

E. faecium LMG 16004c

+

-

+

+

-

-

-

-

+

2.

E. durans LMG 16172c

+

-

-

+

-

-

-

-

+

3.

E. faecium LMG 16192c

+

-

+

+

-

-

-

-

+

4.

E. faecalis LMG 8222c

+

+

-

-

-

-

-

-

+

5.

E. faecalis LMG 17122c

+

+

-

-

-

-

-

-

+

6.

E. faecalis LMG 16216c

+

+

-

-

+

-

-

+

+

7.

E. faecium LMG 16200c

+

-

+

-

-

-

-

-

+

8.

E. raffinosus LMG 12172c

+

-

-

-

-

-

-

-

+

9.

E. mundti LMG 12308c

+

-

-

-

-

-

-

-

+

10.

E. hirae LMG 6399c

+

-

-

-

-

-

-

-

+

11.

E. avium, LMG 10744c

+

-

-

-

-

-

-

-

+

12.

E. faecium BM 4339 d

+

-

+

-

-

-

+

+

+

13.

E. casseliflavus e

+

-

-

-

-

+

-

-

+

14.

S. aureus f

-

-

-

-

-

-

-

-

+

15.

Streptococcus spp. Group Af

-

-

-

-

-

-

-

-

+

16.

Streptococcus spp. Group Bf

-

-

-

-

-

-

-

-

+

17.

Streptococcus spp. Group Gf

-

-

-

-

-

-

-

-

+

18.

Streptococcus spp. Group Ff

-

-

-

-

-

-

-

-

+

19.

Bacillus spp.f

-

-

-

-

-

-

-

-

+

20.

Listeria spp.f

-

-

-

-

-

-

-

-

+

21.

Corynebacterium spp.f

-

-

-

-

-

-

-

-

+

22.

Gardnerella spp.f

-

-

-

-

-

-

-

-

+

23.

Lactobacillus spp.f

-

-

-

-

-

-

-

-

+

24.

E. coli (EHEC)f

-

-

-

-

+

-

-

-

+

25.

E. coli (EPEC)f

-

-

-

-

-

-

-

-

+

26.

E. coli (ETEC)f

-

-

-

-

+

-

-

-

+

27.

V. cholerae (O1 classical)f

-

-

-

-

-

-

-

-

+

28.

V. cholerae O139 f

-

-

-

-

-

-

-

-

+

29.

V. mimicus f

-

-

-

-

-

-

-

-

+

30.

V. cincinnatiensis f

-

-

-

-

-

-

-

-

+

31.

V. furnissii f

-

-

-

-

-

-

-

-

+

32.

V. parahaemolyticus f

-

-

-

-

-

-

-

-

+

33.

S. typhi f

-

-

-

-

-

-

-

-

+

34.

S. sonnei f

-

-

-

-

-

-

-

-

+

35.

S. enterica f

-

-

-

-

-

-

-

-

+

36.

S. dysenteriae f

-

-

-

-

-

-

-

-

+

37.

S. boydii f

-

-

-

-

-

-

-

-

+

38.

C. freundii f

-

-

-

-

-

-

-

-

+

39.

Y. enterocolitica f

-

-

-

-

-

-

-

-

+

40.

P. mirabilis f

-

-

-

-

-

-

-

-

+

41.

P. aeruginosa f

-

-

-

-

-

-

-

-

+

42.

K. pneumoniae f

-

-

-

-

-

-

-

-

+

43.

P. Shigelloides f

-

-

-

-

-

-

-

-

+

  1. aEnterococcus genus
  2. b Bifunctional aminoglycoside resistant genotype
  3. c Reference strains from Belgian Co-ordinated Collections of Micro-organisms (BCCM), Ghent, Belgium
  4. d Kindly provided by Professor. Patrice Courvalin and Dr. Bruno Perichon, Institut Pasteur, Paris, France
  5. e Obtained from Institute for Medical Research, Malaysia
  6. f Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia. '+' is positive; '-' is negative by V-BiA-RE PCR
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BMC Microbiology

ISSN: 1471-2180

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