Identification of genomic differences between Campylobacter jejuni subsp. jejuni and C. jejuni subsp. doylei at the nap locus leads to the development of a C. jejuni subspeciation multiplex PCR method

Table 1 nap and lpxA multiplex primers

nap mpx1:
napAL2	Flanking	5' CTT TAG AAG GGC TTT TAG CTC GTG C 3'
napAR4	Flanking	5' ATT TCC CTG CAA GAT AAA ATC TGT AGC 3'
napBIF	Internal	5' AGA AAA GCA AGT TTA GAA AAT GAA AAT AA 3'
napBIR	Internal	5' GCA TCA CTT TGT GGA ACA TGA CA 3'
*nap* mpx2:
napAIF3	Internal	5' TAG AAC AAA TAA TAT CGA TCC AAA TGC 3'
napAIR3	Internal	5' AAA AGT GTA TCA TCT TCG CTA TAA CCC 3'
napBL	Flanking	5' GGA ATG ATA CAT AGA GGG ATT ATT TTT G 3'
napBR	Flanking	5' AAT TTC ACC TTT ATC AGT GCC TAT ATA 3'
*lpxA*:
KK2		5' CAA TCA TGW GCN ATA TGR CAA TAN GCC 3'
lpxAC. coli		5' AGA CAA ATA AGA GAG AAT CAG 3'
lpxAC. jejuni		5' ACA ACT TGG TGA CGA TGT TGT A 3'
Cl0122		5' CTT ACC AAA TGT TAA AAT AGG C 3'
lpxAC. upsaliensis		5' AAG TCG TAT ATT TTC YTA CGC TTG TGT G 3'

lpxAC. coli, lpxAC. jejuni and lpxAC. upsaliensis are from Klena et al. [17]. KK2 and Cl0122 are derived from lpxARKK2m and lpxAC. lari, respectively [17]; the Cl0122/KK2 amplicon is 2 bp longer than the respective lpxARKK2m/lpxAC. lari amplicon.

ISSN: 1471-2180