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Table 1 16S variable region range definitions.

From: Bacterial flora-typing with targeted, chip-based Pyrosequencing

Variable region E. coli 16S rDNA range 5' primer 3' primer
  start end length   
V1 8 120 113 5'-AGAGTTTGATCMTGGCTCAG 5'-TTACTCACCCGTICGCCRCT
V2 101 361 261 5'-AGYGGCGIACGGGTGAGTAA 5'-CYIACTGCTGCCTCCCGTAG
V3 338 534 197 5'-ACTCCTACGGGAGGCAGCAG 5'-ATTACCGCGGCTGCTGG
V4 519 806 288 5'-TGCCAGCAGCCGCGGTAA 5'-GGACTACARGGTATCTAAT
V5 787 926 140 5'-ATTAGATACCYTGTAGTCC 5'-CCGTCAATTCMTTTGAGTTT
V6 907 1073 167 5'-AAACTCAAAKGAATTGACGG 5'-ACGAGCTGACGACARCCATG
V7 & VV8 1054 1406 353 5'-CATGGYTGTCGTCAGCTCGT 5'-ACGGGCGGTGTGTAC
V9 1392 1507 116 5'-GTACACACCGCCCGT 5'-TACCTTGTTACGACTT
  1. Regions were chosen to be mostly non-overlapping, each containing one or two variable regions. Coordinates are given relative to the 1542 bp E. coli K12 16S rDNA sequence.