Figure 2

RT-PCR using S. schenckii yeast and mycelium RNA of a conserved region in sscmk1 gene. Total RNA was extracted from yeast and mycelium cells of S. schenckii and used as templates for RT-PCR using the DASQTI/WSMGVI primer pair. Fifteen μl of each reaction were resolved in a 1.2% agarose gel electrophoresis. Lanes 3 and 6, show the results obtained from the RT-PCR using total RNA extracted from yeast and mycelium cells respectively, and show the 279 bp band. Lanes 2 and 5 represent the RT-PCR control for each time point where no M-MLV reverse transcriptase was added. Lanes 4 and 7 show a 363 bp product corresponding to a control PCR reaction using genomic DNA as template and the same primer pair. Lane 1 shows the 123 bp DNA Ladder. The position of each of the RT-PCR products is indicated by arrows.