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Figure 3 | BMC Microbiology

Figure 3

From: DprA/Smf protein localizes at the DNA uptake machinery in competent Bacillus subtilis cells

Figure 3

Change in the localization pattern of Smf-YFP. A-D) Strain ST21 (smf-yfp) grown to competence, A) without addition of DNA, B) 10 minutes after addition of chromosomal DNA, C) 20 minutes after addition of DNA, D) 30 minutes after addition of DNA; E) strain ST33 (smf-yfp, comGA-cfp) grown to competence, 20 min after addition of DNA, white triangles indicate cells containing ComGA-CFP foci, but no polar Smf-YFP foci, F) strain ST35/comEC::Ery, smf-yfp) grown to competence, 20 minutes after addition of DNA. G) strain ST36 (recA::Cm, smf-yfp) grown to competence, 20 minutes after addition of DNA. White lines indicate ends of cells (where these are not easily seen). All images were equally scaled; grey bars 2 μm.

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