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Table 2 Primers used in this study

From: Improved protocols for functional analysis in the pathogenic fungus Aspergillus flavus

Primer name

Sequence*

Purpose**

P0

TTGAAGTCATGGTGGAGGTG

1

P1

ATGTACTCCGTACTCGGTTG

1, 2

P2

TTATATTCTCCCTCGCCATActtggaccacacgagtcaag

1, 2

P3

cttgactcgtgtggtccaagTATGGCGAGGGAGAATATAA

1, 2

P4

gcggaggatatttcgttttcATAACAAGGCCTCGTATGTG

1, 2

P5

CACATACGAGGCCTTGTTATgaaaacgaaatatcctccgc

1, 2

P6

ACTACACCTTGGGGACTGTA

1, 2

  1. * Sequence in upper-case is homologous to A. flavus genome, and lower-case is homologous to pyr4 gene of N. crassa.
  2. ** Purpose 1: identifying transformants; purpose 2: constructing overlap PCR construct.
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