Figure 7
From: Improved protocols for functional analysis in the pathogenic fungus Aspergillus flavus
PCR-amplification of 2 uracil-independent transformants SOD#5 and SOD#30 using different primer pairs to confirm if the transformation construct replaced the target sequence. Lane 2–4: template DNA from transformant SOD#5 (Lane 3 in Figure 6), Lane 5–7: template DNA from transformant SOD#30 (lane 12 in Figure 6). Primer pairs used as: P1 and P6 were used on lane 2 and 5, band size is 3385 bp; Primers P0 and P6 were used on lane 3 and 6, band size is 3385 + 101 = 3486 bp; Primers P0 and P5 were used on lane 4 and 7, band size is 3385 + 101 -- 1005 = 2481 bp.