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Table 3 Oligonucleotides used in this study

From: Identification and regulation of expression of a gene encoding a filamentous hemagglutinin-related protein in Bordetella holmesii

Oligonucleotide Sequence (5'-3')* Restriction sites
Fha1F 5'-CTCATCATCGCCAACCCCAACGG -3' -
Fha1R 5'-AGCTGGCGCACGCCCAGGCCTG -3' -
Fha2F 5'-CCCAAGCCCAAGCCCAAGCCCAAGGCC -3' -
Fha2R 5'-ATAGAAGACCCGGTAGTTCT -3' -
FhaBamHI 5'-CCTCGGAGGATCC CCTCCATCGA -3' BamHI
FhaHindIII 5'-TACTTTGCTGAAGCTT AAACGATAG -3' HindIII
Fhagfp1 5'-CCTCGGAGGATCC CCTCCATCGA -3' BamHI
Fhagfp2 5'-ACAACGAGAGGATCC GCAGCAA -3' BamHI
Fhagfp3 5'-CAAAAGGGGATCC ACGGGGCAA -3' BamHI
Fhagfp4 5'-AGGGTGCGAGGATCC TGACACA -3' BamHI
Fhagfp6 5'-AAGTGTTGGGATCC GTAGTGTCT -3' BamHI
Fhagfp7 5'-AACGATCTAGA TCCGCGCTGCCC -3' XbaI
FhaGR1 5'-GACTATCCTGACACATTGAGGAG -3' -
FhaGR2 5'-CTACATGTAAGTAGGGCCCTGTG -3' -
Gfp1 5'-CAAGAATTGGGACAACTCCAGT -3' -
  1. * Restriction sites introduced for cloning purposes are underlined.