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Figure 8 | BMC Microbiology

Figure 8

From: Identification and regulation of expression of a gene encoding a filamentous hemagglutinin-related protein in Bordetella holmesii

Figure 8

Immunoblot analysis of protein lysates of B. holmesii strains with a polyclonal anti-GFP antiserum. B. holmesii G7702 (pMMB208-fhaP-gfp0; BS1 to BS4) (lane 1), B. holmesii G7702 bvgA (pMMB208-fhaP-gfp0; BS1 to BS4) (lane 2), B. holmesii G7702 (pMMB208-fhaP-gfp2; BS2 to BS4) (lane 3), B. holmesii G7702 (pMMB208-fhaP-gfp3; BS3 and BS4) (lane 4), B. holmesii G7702 (pMMB208-fhaP-gfp4; BS4) (lane 5), B. holmesii G7702 (pMMB208-fhaP-gfp6; no BS) (lane 6). Lysates of E. coli (pSK-fhaP-gfp0) expressing GFP under control of the pSK lac promoter (lane 7) and of E. coli (pMMB208-fhaP-gfp0) (lane 9) were analysed as positive and negative control, respectively. BH-WT, B. holmesii wild type; BH-bvgA, B. holmesii bvgA mutant.

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BMC Microbiology

ISSN: 1471-2180

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