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Figure 5 | BMC Microbiology

Figure 5

From: Identification and regulation of expression of a gene encoding a filamentous hemagglutinin-related protein in Bordetella holmesii

Figure 5

Binding of the B. holmesii BvgA BH protein to the fhaB upstream region of B. holmesii. The footprint shows the entire region protected by BvgABH as indicated by the bar on the right side of the figure. DNase I footprint experiments were performed on a 312 bp BamHI-HindIII DNA fragment from plasmid pSK-FP labelled at its BamHI site containing the entire fhaB upstream sequence including all four putative binding sites (BS1–BS4). The 5'-labelled probe was incubated with 0.5, 1.0, 2.0, 3.0, 4.0, 6.0 and 8.0 μg of in vitro phosphorylated BvgABH (lanes 4–10, respectively). No protein was added to the reaction mixture loaded in lane 3. Lane 1 and 2 are G+A sequencing reactions on the DNA probe used as a size marker [42]. Numbers on the left indicate the distance from the translational start codon of the fhaB gene. The positions of the start sites of transcripts P1–P3 are indicated.

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