Figure 4From: Identification and regulation of expression of a gene encoding a filamentous hemagglutinin-related protein in Bordetella holmesiiBinding of BvgA BH to the fhaB upstream region of B. holmesii. A radiolabelled 277 bp PCR fragment containing the fhaB upstream region of B. holmesii was incubated with 150, 350, 500 and 650 ng of unphosphorylated (lanes 1–4) and in vitro phosphorylated (lanes 5–8) BvgABH, respectively. Lane 9 contains the radiolabelled DNA probe. The reaction mixtures were run on a non-denaturating 4% polyacrylamide gel. F, free DNA; C, DNA-protein complex.Back to article page