Table 1 Strains and plasmids used in study.
Name | Phenotype/Relevant Characteristics | Reference or Source |
|---|---|---|
U112 | Francisella novicida prototype strain. | ATCC |
JL0 | U112, Δsucrose hydrolase strain used to make deletion mutants. | Laboratory strain |
ODB2 | JL0, ΔiglA | This study |
ODB7 | U112, iglA::EmR | This study |
ODB1 | U112,iglB::EmR | This study |
ODB5 | ΔiglA/iglA:KmR, in cis complementation of iglA in strain ODB2 | This study |
CG62 | U112, iglC::TnMax 2 | Gray et al. (2002) |
GB2 | U112, mglA | Baron et al. (1998) |
GB6 | U112, mglB::mTn 10 Km | Baron et al. (1998) |
DH5α | F- Φ80dlac ZΔM15 Δ(lac ZYA-arg F)U169 rec A1 end A1 hsd R17(rk -, mk +) pho A sup E44 thi-1 gyr A96 rel A1 λ- | Invitrogen |
pCR2.1 | Cloning vector, AmpR KmR | Invitrogen |