In cis complementation of iglA. Panel A. Diagram of complementation scheme. A PCR amplicon containing the iglA and neighboring regions was ligated to a KmR cassette and used to transform a ΔiglA strain. Integration of the recombinant construct resulted in a strain with a chromosomally-integrated iglA. Panel B. PCR reactions demonstrating the presence of iglA in the complemented strain. Arrows in lower part of diagram indicate the location of the PCR primers used in the reactions.