Figure 5
From: Variable coordination of cotranscribed genes in Escherichia coli following antisense repression
Dose effect of antisense PNA on LacZ protein activity and lacZ mRNA abundance. AS19 cells induced with 100 μM IPTG and treated with increasing concentrations of anti-lacZ PNA were harvested at ΔOD550 < 0.1 for LacZ activity and RT-PCR measurements. (A) Relative amounts of LacZ protein (hatched bars) and lacZ mRNA (slashed bars) are shown as mean values from three separate experiments +/- std. Nontreated samples were used as standard and un-induced samples were used to remove background. (B) Nonlinear correlation between LacZ activity and mRNA abundance after treatment with anti-lacZ PNA at increasing concentrations. The average values shown in panel (A) were plotted following log transformation of the LacZ activity data. Linear regression analysis of the plotted data indicates R = 0.974 for the correlation with the two tailed p < 0.001.