Identification of pathogenic Leptospira species by conventional or real-time PCR and sequencing of the DNA gyrase subunit B encoding gene

Table 4 Oligonucleotides used in this study.

Assay	Use	Oligonucleotide	Sequence (5'–3')	Reference
gyrB – Conventional and real-time PCR.	Amplification and sequencing	2For	TGAGCCAAGAAGAAACAAGCTACA	This study
		504Rev	MATGGTTCCRCTTTCCGAAGA
16s rRNA gene	Amplification and sequencing	FD1MOD	AGAGTTTGATCYTGGYTYAG	[25]
		13R	AGGCCCGGGAACGTATTCAC	[26]
	Sequencing	515F	GTGCCAGCAGCCGCGGTAA	[26]
		91e	TCAAAGGAATTGACGGGGGC	[26, 27]
		11e	GAGGAAGGTGGGGATGACG	[27]
		16s1RRB	CTTTACGCCCARTRAWTCCG	[28]
		907R	CCGTCAATTCCTTTRAGTTT	[29]
		342R	CTGCTGCSYCCCGTAG	[29]

ISSN: 1471-2180