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Table 2 Assignment of strains to the biogroups originally defined by Farmer et al. [1]

From: The biochemical differentiation of Enterobacter sakazakii genotypes

Farmer Biogroup

Additional group

Phenotypea

No. of strains

16s rDNA cluster

  

VP

MR

Nit

Orn

Mot

Ino

Dul

Ind

Malo

Gas

  

1

 

+

-

+

+

+

+

-

-

-

+

60

1

2

 

+

-

+

+

+

-

-

-

-

+

38

1

 

2a

+

-

+

+

-

-

-

-

-

+

4

1

3

 

+

-

+

+

-

+

-

-

-

+

6

1

4

 

+

-

+

-

+

+

-

-

-

+

6

1

 

4a

+

-

+

-

-

+

-

-

-

+

3

1

5

 

+

-

+

+

+

+

-

-

+

+

10

1

 

5a

+

-

+

+

-

+

-

-

+

+

6

1

6

 

+

-

+

+

+

+

-

+

-

+

2

4

7

 

+

-

+

+

+

+

-

-

-

-

4

1

8

 

+

-

-

+

+

+

-

-

-

+

0

1

 

8a

+

-

-

+

+

-

-

-

-

+

3

1

 

8b

+

-

-

+

+

+

-

-

+

+

2

1

 

8c

+

-

-

+

+

-

-

-

+

+

2

1

9

 

+

-

+

+

+

-

-

-

+

+

12

1

 

9a

-

-

+

+

+

-

-

-

+

+

1

1

10

 

+

-

+

+

+

-

-

+

-

+

1

4

11

 

+

-

+

+

+

-

+

-

-

+

1

1

12

 

+

-

+

+

+

+

-

+

+

+

1

4

13

 

-

+

+

+

+

+

-

-

-

+

2

1

 

13a

-

+

+

+

-

+

-

-

-

+

3

1

 

13b

-

+

+

-

+

+

-

-

-

+

1

1

 

13c

-

-

+

+

+

+

-

-

-

+

1

1

14

 

+

-

+

-

+

-

-

-

+

+

4

1

 

14a

+

-

+

-

+

-

-

-

-

+

1

1

15

 

+

-

+

+

+

+

+

+

+

+

6

3

New Biogroup

16

+

-

+

+

+

+

+

-

+

+

6

2

 

16a

+

-

+

+

-

+

+

-

+

+

1

2

 

16b

+

-

+

+

+

+

+

-

-

+

1

2

 

16c

+

-

+

-

-

+

+

-

+

+

1

2

  1. aVP, Voges-Proskauer; MR, methyl red; Nit, nitrate reduction; Orn, ornithine utilization; Mot, motility at 37°C; Ino, acid production from inositol; Dul, acid production from dulcitol; Ind, indole production; Malo, malonate utilization; Gas, gas production from glucose. All isolates positively reacted with α-methyl-D-glu, with the exception of biogroup 15 isolates.