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Table 2 Assignment of strains to the biogroups originally defined by Farmer et al. [1]

From: The biochemical differentiation of Enterobacter sakazakii genotypes

Farmer Biogroup Additional group Phenotypea No. of strains 16s rDNA cluster
   VP MR Nit Orn Mot Ino Dul Ind Malo Gas   
1   + - + + + + - - - + 60 1
2   + - + + + - - - - + 38 1
  2a + - + + - - - - - + 4 1
3   + - + + - + - - - + 6 1
4   + - + - + + - - - + 6 1
  4a + - + - - + - - - + 3 1
5   + - + + + + - - + + 10 1
  5a + - + + - + - - + + 6 1
6   + - + + + + - + - + 2 4
7   + - + + + + - - - - 4 1
8   + - - + + + - - - + 0 1
  8a + - - + + - - - - + 3 1
  8b + - - + + + - - + + 2 1
  8c + - - + + - - - + + 2 1
9   + - + + + - - - + + 12 1
  9a - - + + + - - - + + 1 1
10   + - + + + - - + - + 1 4
11   + - + + + - + - - + 1 1
12   + - + + + + - + + + 1 4
13   - + + + + + - - - + 2 1
  13a - + + + - + - - - + 3 1
  13b - + + - + + - - - + 1 1
  13c - - + + + + - - - + 1 1
14   + - + - + - - - + + 4 1
  14a + - + - + - - - - + 1 1
15   + - + + + + + + + + 6 3
New Biogroup 16 + - + + + + + - + + 6 2
  16a + - + + - + + - + + 1 2
  16b + - + + + + + - - + 1 2
  16c + - + - - + + - + + 1 2
  1. aVP, Voges-Proskauer; MR, methyl red; Nit, nitrate reduction; Orn, ornithine utilization; Mot, motility at 37°C; Ino, acid production from inositol; Dul, acid production from dulcitol; Ind, indole production; Malo, malonate utilization; Gas, gas production from glucose. All isolates positively reacted with α-methyl-D-glu, with the exception of biogroup 15 isolates.