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Table 2 Oligonucleotides utilized in this study

From: Functional characterization of a three-component regulatory system involved in quorum sensing-based regulation of peptide antibiotic production in Carnobacterium maltaromaticum

Primer

Sequence

BRcbnKR-f

5'-TAAAAGCTTTTGAATTCTGATATACTC-3'

BRcbnKR-r

5'-TCTCATCTTGCTGCAGGACAAATG-3'

BRH239A-f

5'-AGGAAATTTAAGGCG GACTATGAGAATC-3'

BRH239A-r

5'-GAGATTCTCATAGTCCGC CTTAAATTTCC-3'

BRD62N-r

5'-TGATTTAAATCGATGTT TAAAAAGTAAATTC-3'

BRBsmFI-f

5'-GTTACTTTAGTATTGGGGACCG-3'

BRBsp-f

5'-GATACAAGGAACTTCTTCGAAAG-3'

BRBsp-r

5'-CTTTCGAAGAAGTTCCTTGTATC-3'

PcbnBM1-f

5'-CCGGGATCC TATGTCCTTTTGAAAAACCAAACTC-3'

PcbnBM1-r

5'-CCGGAATT CGTCTATACACTCCTTATAAAGTATTT3'

  1. Restriction sites introduced with the oligonucleotides are underlined. Mutated codons are shown in bold.