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Table 1 Bacterial strains and plasmids utilized in this study

From: Functional characterization of a three-component regulatory system involved in quorum sensing-based regulation of peptide antibiotic production in Carnobacterium maltaromaticum

Bacterial strain or plasmid Relevant characteristics Reference, source
Strains   
Escherichia coli DH5α Cloning host [25]
Lactococcus lactis MG1363 Gram-positive bacterium [28]
Carnobacterium maltaromaticum LV17B Carnobacteriocin producer carrying pCP40 [29]
Carnobacterium maltaromaticum LV17C C. maltaromaticum LV17B derivative lacking pCP40 [29]
Plasmids   
pUC18 E. coli cloning vector, Ampr [25]
pNZ273 L. lactis promoter screening vector, gusA reporter, CmR [24]
pIL253 L. lactis expression vector, cloned genes are transcribed from the rep promoter, EmR [23]
pK194 E. coli cloning vector, KmR [30]
pLQB14 pK194 carrying the 14-kbp Bam H1 fragment of pCP40 This study
pCbnKR pUC18 with cbnK-cbnR insert, AmpR This study
pBRKR pIL253 expressing cbnK-cbnR, EmR This study
pBRKA239R pBRKR derivative with His 239 of CbnK replaced by Ala, Emr This study
pBRKRN62 pBRKR derivative with Asp 62 of CbnR replaced by Asn, Emr This study
pLQPBM1 pNZ273 with PBM1 promoter upstream of gusA, CmR This study
pLQPB pNZ273 with PB promoter upstream of gusA, CmR [19]
pLQPS pNZ273 with PS promoter upstream of gusA, CmR [19]