Strains
| | |
Escherichia coli DH5α
|
Cloning host
|
[25]
|
Lactococcus lactis MG1363
|
Gram-positive bacterium
|
[28]
|
Carnobacterium maltaromaticum LV17B
|
Carnobacteriocin producer carrying pCP40
|
[29]
|
Carnobacterium maltaromaticum LV17C
|
C. maltaromaticum LV17B derivative lacking pCP40
|
[29]
|
Plasmids
| | |
pUC18
|
E. coli cloning vector, Ampr
|
[25]
|
pNZ273
|
L. lactis promoter screening vector, gusA reporter, CmR
|
[24]
|
pIL253
|
L. lactis expression vector, cloned genes are transcribed from the rep promoter, EmR
|
[23]
|
pK194
|
E. coli cloning vector, KmR
|
[30]
|
pLQB14
|
pK194 carrying the 14-kbp Bam H1 fragment of pCP40
|
This study
|
pCbnKR
|
pUC18 with cbnK-cbnR insert, AmpR
|
This study
|
pBRKR
|
pIL253 expressing cbnK-cbnR, EmR
|
This study
|
pBRKA239R
|
pBRKR derivative with His 239 of CbnK replaced by Ala, Emr
|
This study
|
pBRKRN62
|
pBRKR derivative with Asp 62 of CbnR replaced by Asn, Emr
|
This study
|
pLQPBM1
|
pNZ273 with PBM1 promoter upstream of gusA, CmR
|
This study
|
pLQPB
|
pNZ273 with PB promoter upstream of gusA, CmR
|
[19]
|
pLQPS
|
pNZ273 with PS promoter upstream of gusA, CmR
|
[19]
|