Functional characterization of a three-component regulatory system involved in quorum sensing-based regulation of peptide antibiotic production in Carnobacterium maltaromaticum

Table 1 Bacterial strains and plasmids utilized in this study

Bacterial strain or plasmid	Relevant characteristics	Reference, source
Strains
Escherichia coli DH5α	Cloning host	[25]
Lactococcus lactis MG1363	Gram-positive bacterium	[28]
Carnobacterium maltaromaticum LV17B	Carnobacteriocin producer carrying pCP40	[29]
Carnobacterium maltaromaticum LV17C	C. maltaromaticum LV17B derivative lacking pCP40	[29]
Plasmids
pUC18	E. coli cloning vector, Amp^r	[25]
pNZ273	L. lactis promoter screening vector, gusA reporter, Cm^R	[24]
pIL253	L. lactis expression vector, cloned genes are transcribed from the rep promoter, Em^R	[23]
pK194	E. coli cloning vector, Km^R	[30]
pLQB14	pK194 carrying the 14-kbp Bam H1 fragment of pCP40	This study
pCbnKR	pUC18 with cbnK-cbnR insert, Amp^R	This study
pBRKR	pIL253 expressing cbnK-cbnR, Em^R	This study
pBRKA239R	pBRKR derivative with His 239 of CbnK replaced by Ala, Em^r	This study
pBRKRN62	pBRKR derivative with Asp 62 of CbnR replaced by Asn, Em^r	This study
pLQPBM1	pNZ273 with PBM1 promoter upstream of gusA, Cm^R	This study
pLQPB	pNZ273 with PB promoter upstream of gusA, Cm^R	[19]
pLQPS	pNZ273 with PS promoter upstream of gusA, Cm^R	[19]

ISSN: 1471-2180