A: Expression and purification of recombinant putative six histidine tagged deoxyhypusine synthase from P. vivax by Nickel-chelate chromatography. 12% SDS PAGE protein gel: 1) protein marker: 10 kDa ladder; 2) non induced bacterial cell lysate 3) induced bacterial cell lysate with 100 mM IPTG after 4 hours of induction; 4 and 5) wash fractions obtained after purification; 5 and 6) eluate fractions of purified putative deoxyhypusine synthase. B: Western Blot analysis of the expressed, purified P. vivax DHS protein. lane 0) standard molecular size marker (Roth); lane 1) protein extract of purified DHS protein from P. vivax by Nickel-chelate chromatography. lane 2) protein extract of purified human DHS protein. The blot was probed with a 1: 800 diluted polyclonal antiserum against human DHS.