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Table 4 Primers and probes in the primary and confirmatory real-time PCR systems.

From: New PCR systems to confirm real-time PCR detection of Mycobacterium avium subsp. paratuberculosis

Oligo Nucleotide sequence 5' annealing position* Product length
MPF 5'-CCG CTA ATT GAG AGA TGC GAT T-3' 139 115
MPR 5'-CCA GAC AGG TTG TGC CAC AA-3' 253  
MP probe 5'-6-FAM-ACC TCC GTA ACC GTC ATT GTC CAG ATC A-BHQ-1-3' 231  
DH1F 5'-AAG ACC GAC GCC AAA GAC-3' 536 69
DH1R 5'-CAG AGG CTG CAA GTC GTG-3' 604  
DH1 probe 5'-6-FAM-GAT CAT CGC CGA TCA GGC CC-BHQ-1-3' 559  
DH2F 5'-GCC TTC GAC TAC AAC AAG AGC-3' 737 65
DH2R 5'-GCG TCG GGA GTT TGG TAG-3' 801  
DH2 probe 5'-6-FAM-GCC GCG CTG ATC CTG CTT ACT-BHQ-1-3' 761  
DH3F 5'-AAC TAA GCG GAT CGA CAA TTC-3' 493 80
DH3R 5'-TGG TGT ACC GAA TGT TGT TG-3' 572  
DH3 probe 5'-6-FAM-TGC AAC TCG AAC ACA CCT GGG A-BHQ-1-3' 519  
  1. * According to deposited sequences X16293 and X70277 in GenBank