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Table 4 Primers and probes in the primary and confirmatory real-time PCR systems.

From: New PCR systems to confirm real-time PCR detection of Mycobacterium avium subsp. paratuberculosis

Oligo

Nucleotide sequence

5' annealing position*

Product length

MPF

5'-CCG CTA ATT GAG AGA TGC GAT T-3'

139

115

MPR

5'-CCA GAC AGG TTG TGC CAC AA-3'

253

 

MP probe

5'-6-FAM-ACC TCC GTA ACC GTC ATT GTC CAG ATC A-BHQ-1-3'

231

 

DH1F

5'-AAG ACC GAC GCC AAA GAC-3'

536

69

DH1R

5'-CAG AGG CTG CAA GTC GTG-3'

604

 

DH1 probe

5'-6-FAM-GAT CAT CGC CGA TCA GGC CC-BHQ-1-3'

559

 

DH2F

5'-GCC TTC GAC TAC AAC AAG AGC-3'

737

65

DH2R

5'-GCG TCG GGA GTT TGG TAG-3'

801

 

DH2 probe

5'-6-FAM-GCC GCG CTG ATC CTG CTT ACT-BHQ-1-3'

761

 

DH3F

5'-AAC TAA GCG GAT CGA CAA TTC-3'

493

80

DH3R

5'-TGG TGT ACC GAA TGT TGT TG-3'

572

 

DH3 probe

5'-6-FAM-TGC AAC TCG AAC ACA CCT GGG A-BHQ-1-3'

519

 
  1. * According to deposited sequences X16293 and X70277 in GenBank