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Table 2 Primers designed in this study.

From: Construction of a multiple fluorescence labelling system for use in co-invasion studies of Listeria monocytogenes

Primer Sequence
Gfp_for 5'-ATATAGGATCC GAAAGGAGGTTTATTAAAATGGCTAGCAAAGGAGAAGAACTTT-3'
BamHI
Gfp_rev 5'-TTTTTCTGCAG TTATTTGTAGAGCTCATCCATGCC-3'
PstI
HcRed_for 5'-ATATAGGATCC GAAAGGAGGTTTATTAAAATGGTGAGCGGCCTGCTGAAGG-3'
BamHI
HcRed_rev 5'-TTTTTCTGCAG TTAGTTGGCCTTCTCGGGCAGGTC-3'
PstI
DsRedEx_for 5'-ATATAGGATCC GAAAGGAGGTTTATTAAAATGGCCTCCTCCGAGGACGTC-3'
BamHI
DsRedEx_rev 5'-TTTTTCTGCAG TTACAGGAACAGGTGGTGGSGG-3'
PstI
InlA_N_up 5'-ATATAGGATCCGCGGCCGCG AATTTACCTAGTTATACCAATGAAGT-3'
BamHI NotI
InlA_N_down 5'-CATCTAGCTCTTTACACTACTTCTAAGTTCCGCCGGTTTGGGCATC-3'
InlA_C_up 5'-TAGAAGTAGTGTAAAGAGCTAGATG-3'
InlA_C_down 5'-ATATAAAGCTTGCGGCCGC TTCTGCAAAAGCATCATCTGGAAAA-3'
HindIII NotI
  1. Underlined areas mark targets for the indicated restriction enzymes.
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