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Table 1 Strains and plasmids

From: Construction of a multiple fluorescence labelling system for use in co-invasion studies of Listeria monocytogenes

Strains Relevant genotype and characteristics Reference
E. coli.   
S17-1 λ pir thi rspL (Str r) endA sbcB15 sbcC hsdR (rk- mk-) Δ (lac-proAB) [F' traD36 lacIq Δ(lacZ)M15 proA+B+] [26]
JM105 Cloning host. thi rspL (Str r) endA sbcB15 sbcC hsdR (rk- mk-) Δ(lac-proAB) [F' traD36 lacIq Δ (lacZ)M15 proA+B+] [27]
L. monocytogenes EGDe Host strain [28]
L. monocytogenes LO28 Host strain [29]
L. monocytogenes ScottA Host strain [30]
Wt NalR mutant of L. monocytogenes ScottA (wt)  
inlA' NalR; inlA mutant of L. monocytogenes ScottA (wt) carrying an inframe deletion of the last 800 bp of the inlA gene. This study
wt-CFP NalR; EmR; (ScottA wt) harbouring pJEBAN2 This study
wt-GFP NalR; EmR; (wt) harbouring pNF8 This study
wt-YFP NalR; EmR; (ScottA wt) harbouring pJEBAN3 This study
wt-HcRed NalR; EmR; (ScottA wt) harbouring pJEBAN4 This study
wt-DsRed NalR; EmR; (ScottA wt) harbouring pJEBAN6 This study
inlA'-CFP NalR; EmR; (ScottA inlA') harbouring pJEBAN2 This study
inlA'-YFP NalR; EmR; (ScottA inlA') harbouring pJEBAN3 This study
Plasmids   
pUC-cfp++ ApR; donor of cfp++ gene This study
pUC-yfp++ ApR; donor of yfp++ gene This study
PhcRed1 ApR; donor of HcRed gene Clontech laboratories Inc.
PdsRed-Express ApR; donor of DsRed-Express gene Clontech laboratories Inc.
pNF8 EmR, Mob+(IncP), oriR pAMβ1, oriR pUC; Pdlt-gfpmut1 [10]
PJEBAN2 EmR, Mob+(IncP), oriR pAMβ1, oriR pUC; Pdlt-cfp+ This study
PJEBAN3 EmR, Mob+(IncP), oriR pAMβ1, oriR pUC; Pdlt-yfp+ This study
PJEBAN4 EmR, Mob+(IncP), oriR pAMβ1, oriR pUC; Pdlt-HcRed This study
PJEBAN6 EmR, Mob+(IncP), oriR pAMβ1, oriR pUC; Pdlt-DsRedExpress This study
pAUL-A EmR, [24]
pJEBAN12 EmR, pAUL-A carrying a 538 bp PCR product that give rise to an inframe deletion of the last 771 bp of InlA when recombined into the chromosome of L. monocytogenes ScottA This study
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