|
E. coli.
| | |
|
S17-1 λ pir
|
thi rspL (Str r) endA sbcB15 sbcC hsdR (rk- mk-) Δ (lac-proAB) [F' traD36 lacIq Δ(lacZ)M15 proA+B+]
|
[26]
|
|
JM105
|
Cloning host. thi rspL (Str r) endA sbcB15 sbcC hsdR (rk- mk-) Δ(lac-proAB) [F' traD36 lacIq Δ (lacZ)M15 proA+B+]
|
[27]
|
|
L. monocytogenes EGDe
|
Host strain
|
[28]
|
|
L. monocytogenes LO28
|
Host strain
|
[29]
|
|
L. monocytogenes ScottA
|
Host strain
|
[30]
|
|
Wt
|
NalR mutant of L. monocytogenes ScottA (wt)
| |
|
inlA'
|
NalR; inlA mutant of L. monocytogenes ScottA (wt) carrying an inframe deletion of the last 800 bp of the inlA gene.
|
This study
|
|
wt-CFP
|
NalR; EmR; (ScottA wt) harbouring pJEBAN2
|
This study
|
|
wt-GFP
|
NalR; EmR; (wt) harbouring pNF8
|
This study
|
|
wt-YFP
|
NalR; EmR; (ScottA wt) harbouring pJEBAN3
|
This study
|
|
wt-HcRed
|
NalR; EmR; (ScottA wt) harbouring pJEBAN4
|
This study
|
|
wt-DsRed
|
NalR; EmR; (ScottA wt) harbouring pJEBAN6
|
This study
|
|
inlA'-CFP
|
NalR; EmR; (ScottA inlA') harbouring pJEBAN2
|
This study
|
|
inlA'-YFP
|
NalR; EmR; (ScottA inlA') harbouring pJEBAN3
|
This study
|
|
Plasmids
| | |
|
pUC-cfp++
|
ApR; donor of cfp++ gene
|
This study
|
|
pUC-yfp++
|
ApR; donor of yfp++ gene
|
This study
|
|
PhcRed1
|
ApR; donor of HcRed gene
|
Clontech laboratories Inc.
|
|
PdsRed-Express
|
ApR; donor of DsRed-Express gene
|
Clontech laboratories Inc.
|
|
pNF8
|
EmR, Mob+(IncP), oriR pAMβ1, oriR pUC; Pdlt-gfpmut1
|
[10]
|
|
PJEBAN2
|
EmR, Mob+(IncP), oriR pAMβ1, oriR pUC; Pdlt-cfp+
|
This study
|
|
PJEBAN3
|
EmR, Mob+(IncP), oriR pAMβ1, oriR pUC; Pdlt-yfp+
|
This study
|
|
PJEBAN4
|
EmR, Mob+(IncP), oriR pAMβ1, oriR pUC; Pdlt-HcRed
|
This study
|
|
PJEBAN6
|
EmR, Mob+(IncP), oriR pAMβ1, oriR pUC; Pdlt-DsRedExpress
|
This study
|
|
pAUL-A
|
EmR,
|
[24]
|
|
pJEBAN12
|
EmR, pAUL-A carrying a 538 bp PCR product that give rise to an inframe deletion of the last 771 bp of InlA when recombined into the chromosome of L. monocytogenes ScottA
|
This study
|
