Figure 1From: The four serotypes of dengue recognize the same putative receptors in Aedes aegypti midgut and Ae. albopictus cellsVOPBA and DENV2 affinity chromatography of the virus dengue receptors. Proteins from Aedes aegypti MGs were extracted with 0.05% Triton X-100, separated by 10% SDS-PAGE and blotted on to a PVDF membrane. The putative receptors were revealed after incubation with DEN2 virus and peroxidase-labeled goat anti-mouse antibody (panel A, lane 1) as described in the methods section. Receptors were purified from C6/36 cell membranes (panel A, lane 3) or MG (fractions 1 and 2, panel B) by DENV2 affinity chromatography using a DEN2-Sepharose™ 4B column as described in the methods section. The apparent molecular weights of these proteins are shown on the right side of panel A and on the left side of panel B. Proteins separated by SDS-PAGE were silver stained. The protein pattern of total C6/36 membranes is shown in panel A lane 2. Molecular weight markers are shown on the left side in panel A and on the right side in panel B.Back to article page