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Table 4 Determination and characterization of erythromycin and clindamycin MICs and molecular characterization of Brucella eryR mutants.

From: Intrinsic and selected resistance to antibiotics binding the ribosome: analyses of Brucella 23S rrn, L4, L22, EF-Tu1, EF-Tu2, efflux and phylogenetic implications

Brucella ery R strains ERY ERY + PAβN CL CL + PAβN Loci Mutation
B. suis WT 1.5 0.75 8 4 NA  
  a 6 6 12 12 -  
  b 6 6 12 12 ND  
  c 4 6 8 8 -  
  d 6 6 12 12 ND  
  e 6 6 8 8 ND  
B. canis WT 0.5 0.094 8 0.5 NA  
  a 2 0.32 8 1.0 -  
  b 2 0.047 6 <0.016 -  
  c 3 0.047 8 0.38 -  
  d 3 0.047 4 0.125 -  
  e 2 0.19 8 3 -  
B. neotomae WT 0.75 0.047 3 0.75 NA  
  a 4 0.125 8 0.75 -  
  b 3 0.125 6 2 ND  
  c 2 0.064 6 1.5 -  
  d 6 0.25 6 1.0 L22 6 bp indel, β-loop
  e 2 0.38 4 2 -  
Propoise WT 1.5 0.25 16 3 NA  
  a 16 0.75 4 3 L4 G209A; Gly70Asp
  b >256 >256 >256 >256 23S A2058G (Ec)
  c 192 1.5 4 3 L4 Δ18aa (54–71)
  d 48 0.38 6 3 L4 G209A; Gly70Asp
  e 128 1.5 4 3 L4 Δ30aa (54–81)
Seal WT 6 1.5 >256 48 NA  
  a 24 12 96 96 -  
  b 32 24 >256 >256 -  
  c >256 >256 16 16 L4 C217A; Arg73Ser
  d 128 16 >256 >256 -  
  e 128 24 >256 >256 ND  
Dolphin WT 3 0.38 2 0.75 NA  
  a >256 128 1.0 1.5 L4 G209A; Gly70Asp
  b >256 6 1.5 1.0 L4 C217T; Arg73Cys
  c >256 192 1.5 1.5 L4 G209A; Gly70Asp
  d >256 8 2.0 1.0 L4 C217T; Arg73Cys
  e >256 8 1.5 1.0 ND  
  1. Mutants examined were isolates of B. suis biovar 1, B. canis, B. neotomae, and marine porpoise, seal and dolphin strains as per Table 1; WT, wild type; Ery, erythromycin; CL, clindamycin, PAβN, efflux inhibitor L-phenylalanine-L-arginine-β-naphthylamide; -, no mutation identified in any of three ribosomal loci examined; NA, not applicable; ND, sequence not determined.