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Figure 4 | BMC Microbiology

Figure 4

From: Identification and characterization of the heme-binding proteins SeShp and SeHtsA of Streptococcus equi subspecies equi

Figure 4

Reconstitution of holoSeShp (A) and holoSeHtsAHis (B) by hemin. Purified apoSeShp and 82% apoSeHtsHis were incubated with hemin and separated from free hemin by gel filtration using a Sephadex G-25 column. Displayed are the optical absorption spectra of the untreated (dashed curves) and treated (solid curves) protein samples. Concentrations of SeShp and SeHtsAHis were 10 μM and 13 μM, respectively.

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