Gene expression analysis indicates extensive genotype-specific crosstalk between the conjugative F-plasmid and the E. coli chromosome

Table 1 Functional groups of genes differentially expressed between hosts.

Gene functional type	MG1655	Fold-change^#	DH5α	Fold-change^#
Amino acid biosynthesis*	11	5.93^a)
Carbon utilization	8.5	10.23^a)	1	24.10^b)
Chemotaxis			4.5	24.33^b)
Energy production	2.5	3.00^a)	4	3.02^b)
Transposon-related	4.5	3.90^a)	9	172.50^b)
Fatty acid biosynthesis	4	3.95^a)
Flagellum			9.5	28.94^b)
Information transfer	8	3.24^a)	5	9.37^b)
Metabolism general	3	107.20^a)	5	3.13^b)
Unknown	18.5	3.30^a)	8	8.77^b)
Nucleotide biosynthesis	10.5	6.40^a)	2	7.92^b)
Pilus (type I)	2	3.22^a)
Transport	2.5	2.49^a)	2	2.48^b)

Number and average fold-change of genes, up-regulated in DH5α relative to MG1655 (right two columns) and number and average absolute fold-change of genes up-regulated in MG1655 relative to DH5α (left two columns).
*affected amino acids: isoleu/val, gly, try, phe, arg, asp, tyr, thr/ser.
# average among regulatory units within given functional group,
a): higher expression in MG1655 b): higher expression in DH5α.

ISSN: 1471-2180