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Table 1 Effect of inhibitors on Synd1 ectodomain shedding from NMuMG cells enhanced by B. anthracis proteins

From: Acceleration of epithelial cell syndecan-1 shedding by anthrax hemolytic virulence factors

Inhibitors Shed Synd1 (mean % of no-inhibitor control ± C.I.)
  Spontaneous ClnA AnlB AnlO LT
No inhibitor 100 ± 11 100 ± 9 100 ± 12 100 ± 22 100 ± 1
Tyrphostin A25      
5 μM
50 μM
86 ± 6
72 ± 7
100 ± 9 85 ± 17 73 ± 23
67 ± 7
97 ± 8
81 ± 4
Piceatannol      
0.5 μM
5 μM
50 μM
191 ± 31
112 ± 24
46 ± 4
17 ± 9 6 ± 2 136 ± 30
88 ± 25
14 ± 9
199 ± 15
79 ± 12
25 ± 2
Suramin      
20 μM
200 μM
160 ± 27
36 ± 4
89 ± 17 118 ± 7 155 ± 26
25 ± 4
144 ± 14
34 ± 4
PD98059      
5 μM
50 μM
130 ± 10
133 ± 4
103 ± 15 88 ± 3 84 ± 5
56 ± 5
90 ± 4
77 ± 4
SB202190      
5 μM
50 μM
97 ± 10
91 ± 10
108 ± 17 101 ± 19 72 ± 1
39 ± 1
69 ± 4
37 ± 5
JNK inhibitor II      
1.5 μM
15 μM
125 ± 9
145 ± 9
92 ± 13 129 ± 31 89 ± 5
90 ± 5
109 ± 7
103 ± 3
  1. NMuMG cells in 1% FCS medium were preincubated with the indicated concentrations of inhibitors for 1 h, and then exposed to shedding inducers (1 μg/ml of either ClnA, AnlB or LT; 0.1 μg/ml AnlO) for 24 h. Data are expressed relative to shedding observed without inhibitors in cells either treated or untreated with shedding inducers. Confidence intervals correspond to P = 0.05.