Acceleration of epithelial cell syndecan-1 shedding by anthrax hemolytic virulence factors

BMC Microbiology

Table 1 Effect of inhibitors on Synd1 ectodomain shedding from NMuMG cells enhanced by B. anthracis proteins

Inhibitors	Shed Synd1 (mean % of no-inhibitor control ± C.I.)
	Spontaneous	ClnA	AnlB	AnlO	LT
No inhibitor	100 ± 11	100 ± 9	100 ± 12	100 ± 22	100 ± 1
Tyrphostin A25
5 μM 50 μM	86 ± 6 72 ± 7	100 ± 9	85 ± 17	73 ± 23 67 ± 7	97 ± 8 81 ± 4
Piceatannol
0.5 μM 5 μM 50 μM	191 ± 31 112 ± 24 46 ± 4	17 ± 9	6 ± 2	136 ± 30 88 ± 25 14 ± 9	199 ± 15 79 ± 12 25 ± 2
Suramin
20 μM 200 μM	160 ± 27 36 ± 4	89 ± 17	118 ± 7	155 ± 26 25 ± 4	144 ± 14 34 ± 4
PD98059
5 μM 50 μM	130 ± 10 133 ± 4	103 ± 15	88 ± 3	84 ± 5 56 ± 5	90 ± 4 77 ± 4
SB202190
5 μM 50 μM	97 ± 10 91 ± 10	108 ± 17	101 ± 19	72 ± 1 39 ± 1	69 ± 4 37 ± 5
JNK inhibitor II
1.5 μM 15 μM	125 ± 9 145 ± 9	92 ± 13	129 ± 31	89 ± 5 90 ± 5	109 ± 7 103 ± 3

NMuMG cells in 1% FCS medium were preincubated with the indicated concentrations of inhibitors for 1 h, and then exposed to shedding inducers (1 μg/ml of either ClnA, AnlB or LT; 0.1 μg/ml AnlO) for 24 h. Data are expressed relative to shedding observed without inhibitors in cells either treated or untreated with shedding inducers. Confidence intervals correspond to P = 0.05.

ISSN: 1471-2180