Figure 6
M. leprae upreglates Mcl-1 mRNA in THP-1 cells. THP-1 monocytes were infected with M. leprae or BCG at MOI-10 and RNA was harvested after 18 and 48 h of culture. mRNA was reverse transcribed and used for real-time PCR quantification of Mcl-1 and HuPO genes. Relative quantification data of 3 independent experiments calculated using the CT method is illustrated.